نوع مقاله : زیست شناسی (جانوری)
نویسندگان
1 دانشگاه آزاد اسلامی واحد ابهر, ایران
2 دانشکده پزشکی، دانشگاه علوم پزشکی و خدمات بهداشتی , تهران، ایران
3 دانشگاه آزاد اسلامی واحد کرج, ایران
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
Cryosectioning has gained prominence in comparison to classical fixation، processing and paraffin wax embedding of tissue blocks، since many immunolabelling procedures، as well as routine hematoxylin and eosin (H&E) stains، can be performed on cryosections. However، in this method، the tissues would not be preserved for long time. Therefore، choosing and using of a chemicalfixator، which give the best results can be useful for solving the problem. The aim of this study is determination of current fixator effects on cryosections. For this reason، three kinds of current chemical fixatives substances such as acetone، formalin، and formaldehyde were evaluated for their effects on liver cryosections. Samples which used for acetone evaluation، were cut without fixation. But they were fixed for 10 minute after sectioning. Formalin and paraformaldehyde fixed samples were cut after fixation. All sections were stained by hematoxyline and eosin and were studied by light microscope. Paraformaldehyde fixed samples had better results in both preservation and staining quality rather than other fixatives. So، we concluded that for fixation of cryosections، paraformaldehyde is the best choice among formalin or acetone for H&E staining in light microscopy level.
کلیدواژهها [English]
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