ارتباط چندشکلی ژن PIT-I با صفات مرتبط با شیر در گاوهای نر هلشتاین ایران

نوع مقاله : ژنتیک

نویسندگان

1 گروه علوم دامی، دانشکده کشاورزی و دامپزشکی، واحد شبستر، دانشگاه آزاد اسلامی، شبستر، ایران

2 گروه علوم دامی، دانشکده کشاورزی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران

چکیده

این تحقیق به­ منظور تعیین ارتباط چندشکلی ژن عامل رونویسی خاص هیپوفیز یک  (Pit-1) با ارزش­ های ژنتیکی صفات تولید شیر در گاوهای هلشتاین ایران انجام گرفت. بعد از دریافت اطلاعات ارزش­ های اصلاحی از مراکز اصلاح نژاد کشور، با استفاده از نمونه‌های خون 110 رأس گاو نر پروف شده یا در انتظار پروف از دو مرکز اصلاح نژاد شمال­ غرب و تهران، ژنوتیپ افراد با روش هضم آنزیمی (PCR-RFLP) و آنزیم محدودالاثر HifI برای جایگاه موجود در اگزون ششم تعیین گردید. تجزیه و تحلیل داده ­ها با استفاده از مدل مناسب و نرم ­افزار SAS انجام گرفت. در این بررسی دو الل (برش‌ یافته G و برش‌ نیافته A) و سه ژنوتیپ مشاهده  شد به ­طوری که الل‌ (Hinf1 +(G و ژنوتیپ‌ GG فراوان ­ترین بودند. تغییرات فراوانی اللی و ژنوتیپی در طی سال­ های مختلف تولد حیوانات روند مشخصی را نشان نداد و در اکثر سال­ ها برتری با الل G بود. نتایج تجزیه واریانس نشان داد که ارتباط چندشکلی در جایگاه Pit-I-HinfI با تغییرات ارزش ژنتیکی صفات تولید شیر، تولید چربی، تولید پروتئین، درصد چربی و درصد پروتئین معنی‌دار نیست (0/05<p). جایگزینی اللی نشان داد که در صورت تغییر الل G به A تاثیر معنی­ داری در هیچ­ یک از صفات مورد مشاهده نخواهد شد. به ­نظر می ­رسد برای استفاده از چندشکلی‌ ژن Pit-I در بهبود ژنتیکی صفات تولید شیر، نیاز است  تحقیقات بیش ­تری انجام ‌شود.

کلیدواژه‌ها

عنوان مقاله [English]

Relationship of PIT1 gene polymorphism with dairy traits of Iranian Holstein bulls North West and Karaj breeding centers

نویسندگان [English]

  • Abolfazl Gorbani 1
  • Saeed Prnemat Khanegah 2
  • Naser Maheri Sis 1
  • Mahdi Taghinejad Rodbaneh 2
1 Animal Science department, Faculty of agriculture and veterinary, Shabestar Branch, Islamic Azad university, Shabestar, Iran.
2 Department of Animal Science, Faculty of Agriculture, Tabriz Branch, Islamic Azad University, Tabriz, Iran
چکیده [English]

The aim of this study was to determine to Pituitary-specific transcription factor (Pit1) gene polymorphism relationship with milk production in Iranian Holstein dairy cows. For this purpose, blood samples were collected from 110 proof and un-proof Holstein bulls from North West and Tehran breeding centers of Iran. After performing extraction steps using modified phenol-chloroform method, PITI exon 6 polymorphism was determined with PCR-RFLP and HifI restriction enzyme. Two alleles and three genotypes were observed. Hinf1+ (G) allele and GG genotype were the more frequent. Allelic and genotypic variations in years do not show a specific trend, and for most of the years, the G allele was more frequent. The results of analysis of variance showed that PitI-HinfI polymorphism was not significant effect on milk, fat, protein production, fat percentage and protein content (p>0.05). Allelic replacement effect results showed that allele change from G to A did not have a significant effect on any of the studied traits. Based on the results of this study, it can be concluded that for the use of this gene in the of milk production traits improvement, further research is needed.

کلیدواژه‌ها [English]

  • polymorphism
  • PIT1 gene
  • Production trait
  • Holstein

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فصلنامه محیط زیست جانوری
دوره 12، شماره 1
فروردین 1399
صفحه 59-64

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