نوع مقاله : بیماری ها
نویسندگان
1 گروه بهداشت و بیماری های آبزیان، دانشکده دامپزشکی، دانشگاه شیراز، شیراز، ایران
2 گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه شیراز، شیراز، ایران
3 گروه تشخیص بیماری های آبزیان، مرکز ملی تشخیص، آزمایشگاههای مرجع و مطالعات کاربردی، سازمان دامپزشکی کشور، تهران، ایران
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
Viral hemorrhagic septicemia is one of the most important virus disease in salmonid fishes. The genome of the virus consists of six genes, the most important of which is the G glycoprotein gene, which plays a role in immunization against the virus. The aim of this study was detection of viral hemorrhagic septicemia virus in Rainbow trout, phylogenic study and cloning of the G gene of virus in pTZ57r/t vector. Methods: After detecting of viral hemorrhagic septicemia virus by RT-PC, cloning of G gene in pTZ57r/t plasmid, the recombinant plasmid was transformed to the competent bacterium (E. coli). White clonies containing recombinant vector were used to extract the plasmid. The size of the G gene of viral hemorrhagic septicemia virus was 1523 bp. In this study, colony PCR method and sequencing were used to confirm the cloning. Results: The results of this study showed that G gene has been cloned successfully in pTZ57r/t vector and can be used to express the glycoprotein of the virus in expression vectors for protein production. The results of sequencing of G gene also showed that the virus identified in this study belonged to genotype Ia-2.
کلیدواژهها [English]