اثرات سطوح مختلف پروبیوتیک و شکل فیزیکی خوراک بر ریخت شناسی روده باریک و وضعیت ایمنی ‏در جوجه‌های بلدرچین گوشتی

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه علوم دامی، واحد کاشمر، دانشگاه آزاد اسلامی، کاشمر، ایران

چکیده

این آزمایش باهدف استفاده از اثر سطوح مختلف پروبیوتیک پروتوکسین و شکل فیزیکی خوراک بر ریخت‌شناسی روده باریک و وضعیت ایمنی ‏در بلدرچین انجام گرفت. به این منظور تعداد‏ ‏240  قطعه جوجه بلدرچین‌های گوشتی یک روزه در یک آزمایش فاکتوریل و در قالب طرح کاملاً تصادفی ‏در 6 تیمار ، ‏‏٤ تکرار با 10 جوجه در هر تکرار به مدت 42 روز مورد بررسی قرار گرفتند. تیمارهای آزمایشی شامل: 1- خوراک آردی (مش) حاوی آب خالص (فاقد پروبیوتیک پروتکسین)، 2- خوراک آردی (مش) حاوی دو سطح پروبیوتیک پروتکسین محلول در آب آشامیدنی (1 و 2 درصد)، 33- خوراک کرامبل با سه سطح پروبیوتیک پروتکسین (صفر، 1 و 2 درصد) محلول در آب آشامیدنی بودند. جیره‌های آزمایشی بر اساس احتیاجات غذایی جوجه بلدرچین انجمن تحقیقات ‏ملی و با استفاده از نرم‌افزار ‏UFFDA‏ تنظیم شدند. به منظور آزمایش شمارش سلول‌های خونی،  در سن 42 روزگی، یک جوجه از هر تکرار انتخاب و نمونه خون از ورید بال گرفته شد. پس از ثبت وزن تمام جوجه‌ها، یک پرنده از هر قطعه به صورت تصادفی کشتار شد و وزن و طول نسبی بخش‌های مختلف روده و خصوصیات ریخت‌شناسی ژژنوم (‏طول و عرض پرز و عمق کریپت) مورد مطالعه قرار گرفت. ‏نتایج حاصل نشان داد. جوجه‌های تغذیه شده با جیره خوراک کرامبل و فاقد پروبیوتیک پروتکسین دارای بالاترین درصد لنفوسیت و جوجه‌های دریافت کننده خوراک مش و فاقد پروبیوتیک پروتکسین دارای بالاترین درصد مونوسیت بودند (0/05>P). هم چنین نتایج حاصل از ریخت‌شناسی روده باریک جوجه‌های بلدرچین‌ نشان داد که دو تیمار خوراک کرامبل و حاوی 2 درصد پروبیوتیک پروتکسین محلول در آب آشامیدنی دارای بالاترین مقادیر طول پرز و عمق کریپت بودند (0/05>P). وزن نسبی اجزای مختلف دستگاه گوارش و طول نسبی دئودنوم و سکوم به‌طور معنی‌داری تحت تأثیر جیره‌های آزمایشی قرار گرفتند (0/05>P). به‌طورکلی نتایج حاصل از آزمایش نشان داد که استفاده از افزودنی پروبیوتیک پروتکسین منجر به بهبود وضعیت ایمنی بلدرچین‌ها می‌گردد و‏ تأثیر معنی‌داری بر بهبود  وضعیت طول و عرض پرز و عمق کریپت و نیز بهبود وزن و طول نسبی اجزای مختلف روده دارد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Effect of different levels of probiotics and physical form of feed on small intestinal morphology and immune system in Japanese quail chicks

نویسندگان [English]

  • Nasir Sajjadi
  • Reza Vakili
Department of Animal Science, Kashmar Branch, Islamic Azad University, Kashmar, Iran
چکیده [English]

An experiment was conducted to investigate the effect of different levels of probiotics and physical form of feed on the morphology of the small intestine and the immune system in Japanese quail. 240 one-day-old Japanese quail chicks were examined in a factorial experiment in a completely randomized design with 6 replications, 6 replications with 10 chicks per replication for 42 days. Experimental treatments include: Flour feed (mesh) containing pure water (no probiotic proxin), Flour feed (mesh) containing two levels of probiotic soluble protoxin in drinking water (1 and 2%), Crumble feed with three levels of probiotic proxin (0%, 1%) Were soluble in drinking water. Experimental diets were adjusted based on the nutritional needs of quail chicks of the National Research Association using UFFDA software. To test for blood cell count, at 42 days of age, one chick was selected from each replicate and a blood sample was taken from a wing vein. One bird was killed from each pen, and the relative weight and length of different parts of the intestine, the length and width of the villi, and the depth of the crypt were studied. Chickens fed crumble diet and lacking probiotic protexin had the highest percentage of lymphocytes and chickens fed mesh and lacking probiotic protexin had the highest percentage of monocytes (P<0.05). The results of intestinal morphology showed that two treatments of crumble and containing 2% probiotic soluble in drinking water had the highest values ​​of villi length and crypt depth (P<0.05). The relative weights of the various components of the gastrointestinal tract and the relative lengths of the duodenum and cecum were significantly affected by the experimental diets (P<0.05). The results of the experiment showed that the use of probiotic protexin improved the immune system of the Japanese quail and improved the length and width of the villi; The crypt depth as well as the weight and relative length of the various intestinal components were improved.

کلیدواژه‌ها [English]

  • Protexin probiotic
  • Immune status
  • Water science morphology
  • Quail

مراجع

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فصلنامه محیط زیست جانوری
دوره 14، شماره 2
تیر 1401
صفحه 123-130

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