نوع مقاله : مقاله پژوهشی
نویسندگان
1 گروه علوم پایه، دانشکده دامپزشکی، دانشگاه تهران، تهران، ایران
2 گروه پاتوبیولوژی، دانشکده پیرادامپزشکی، دانشگاه بوعلی سینا، همدان، ایران
3 گروه علوم درمانگاهی، دانشکده پیرادامپزشکی، دانشگاه بوعلی سینا، همدان، ایران
چکیده
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Argyrosomus hololepidotus is one of the most valuable fish in Persian Gulf, Oman Sea, and coastal waters of Khuzestan province in the southwest of Iran. The skin, especially in fish, is the first defense line against the external environment and facilitates normal physiological functions in the body. In fish, the epidermal cells of the skin are mainly formed by the division and differentiation of the epidermal epithelial cells. In the present study, for the first time, the surface cell structure of A. hololepidotus was studied using by histology and ESM methods. This study was carried on six A. hololepidotus. For SEM photography, tissue samples of fish skin were fixed by glutaraldehyde 3 % and washed in PBS and post-fixed with osmium tetroxide for 1 h. The specimens were then dehydrated through a graded ethanol series and embedded in resin after primary and post-fixation. The samples were prepared from 5 µm cuts from the fish dorsum and later they were stained by H&E method. The result of the light microscopic and SEM analysis showed that the fish epidermis was composed from goblet, epithelial, and chloride cells. The results of SEM showed that the goblet cells contained mucus droplets. Superficial squamous cells had microridge which chloride cells were accommodated in them. Finally, it can be concluded that the structure of the skin surface cells in Argyrosomus hololepidotus is very similar to the skin of fish of the same family (Sciaenidae).
کلیدواژه‌ها [English]
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