نوع مقاله : زیست شناسی (جانوری)
نویسندگان
1 گروه شیلات، دانشکده علوم کشاورزی و منابع طبیعی، دانشگاه گنبد کاووس، صندوق پستی: 163
2 گروه شیلات، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، صندوق پستی: 487-49175
3 موسسه تحقیقات علوم شیلاتی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، صندوق پستی: 6116- 14155
چکیده
کلیدواژه‌ها
عنوان مقاله [English]
نویسندگان [English]
Eyestalk ablation is the most common procedure to induce gonadic maturation in commercial hatcheries of penaeid shrimp. This study was conducted in order to investigate the potential of using Ovaprim (gonadotropin and dopamine antagonist) hormone to induce ovarian maturation on Litopenaeus vannamei. The female broodstocks were divided into 4 treatments as follows: 1) Eyestalk ablation; 2 and 3) Ovaprim injections at 500 and 1000 ng/g body weight (BW), respectively; 4) sterile vehicle solution injection (control). Reproductive parameters were evaluated such as the number of eggs, oocyte diameters and gonadosomatic indices and number of nauplii. The results of the analysis of reproductive factors showed that there was significant difference between experimental treatments (p< 0.05). The highest average number of egg and nauplii (83100± 16993 and 56132± 5180 respectively) was obtained in eyestalk ablation treatment. The weakest reproductive performance was observed in the sterile vehicle solution injection treatment (control). Hemolymph changs to physiological stress (eyestalk ablation and injection treatments) was investigated. The results showed that humoral cell number was no significant statistical differences between eyestalk ablation and injection treatments (p> 0.05). Metabolic response (protein, glucose, lactate and cortisol) was significantly different between experimental treatments at the time 0 and 45 minutes (p< 0.05). The both time, metabolic parameters increase in the eyestalk ablation treatment and was reduced injection treatment.
کلیدواژه‌ها [English]
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