نوع مقاله : ژنتیک
نویسندگان
1 گروه محیط زیست، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، صندوق پستی: 15739-49138
2 دانشگاه آزاد اسلامی، واحد علوم و تحقیقات، دانشکده محیط زیست و انرژی، گروه زیستگاه ها و تنوع زیستی
چکیده
کلیدواژه‌ها
عنوان مقاله [English]
نویسندگان [English]
The Tufted duck (Aythya fuligula) is one of the most widely distributed of anseriforms. The aim of this study was to analyses the population genetic diversity and structure of the Tufted duckin Gorgan bay with mtDNA genes (Cytochrome b). Extraction of DNA, PCR and DNA sequencing were carried out. Diversity index (Haplotype and nucleotide diversity), Fst index, Gene flow (Nm), exact test, D test of Tajima and Fs test of Fu on 9 sequence were analyzed by DNAsp and Arlequin. Also, we Analysis of the phylogenetic tree and Haplotype network that both draw with our sample and European and Asian population samples existing in Genbank by MEGA, MrBayes and Network. Generally, data set with 961 bp was used for each individual, four haplotypes were obtained, average (±SD) for haplotype diversity was 0.6944 ± 0.147, nucleotide diversity was 0.00095± 0.0008. The high value of Nm (Nm=1.598, P<0.05) among populations based on cytochrome b of mitochondrial gene showed that gene flow between populations were high and genetic differentiation among European and Asian populations and Iran population were low. Mismatch distribution for Tufted duck appeared to be unimodal, which closely matched the expected distributions under the sudden expansion model and supported by the low Harpending’s Raggedness index (0.211). Tajima’s D and Fu’s Fs statistics were 0.715 and -1.282, respectively, and was not significant. The results of this study showed that both European and Asian population of Tufted ducks migrated to Gorgan Bay, so this area should be considered an important area and make a comprehensive plan for management and conservations Measures.
کلیدواژه‌ها [English]
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