نویسندگان
1 گروه زیست شناسی دریا، دانشگاه آزاد اسلامی واحد علوم و تحقیقات تهران، صندوق پستی: 14515-775
2 مرکز تحقیقات نانوتکنولوژی پزشکی و مهندسی بافت، دانشگاه علوم پزشکی شهید بهشتی، تهران
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
The present study was performed to determine toxic effects of nanosilver on Red Blood Cells (RBC) and liver tissue of common carp (Cyprinus carpio). In this study, nanosilver with 10-50 nm in size and concentration of 10, 25, 50 and 100 microgram per liter (PPM) is used. Cellular changes of RBC were studied in Neutral Red Retention Assay (NRR) technique using light microscope at the intervals of 15, 30, 60, 90, 120, 150, and 180 minutes after addition of Neutral Red (NR) to the RBC. Simoultaneously, histopathological changes of the liver tissue were also studied using microscope equipped with a camera and computer. The results were compared with a control group and recorded. The results indicated that the nanosilver with 10 PPM concentration has no toxic effects after the addition of NR on RBC, but in concentration of 25 PPM, cell damage was observed. By changing the concentration of 25 PPM to 50 PPM, destruction of red blood cells increased, so that in the 100 PPM concentration, cell lyses was prominent. Nanosilver with 10 PPM concentration had shown relatively mild toxic effects on liver tissue, while shifting the concentration of nanosilver from 10 PPM to 25, 50 and 100 PPM, resulted in increased liver tissue damage. It may be concluded that, severity of toxic effects of nanosilver on fish RBC and the liver tissue are directly related to increased nanosilver concentration and duration of exposure.
کلیدواژهها [English]
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