Effect of Different Marine Probiotics Individual and with Β-Glucan on Digestive Enzymes Activity, and Intestine Flora of Acanthopagrus Arabicus

Abstract

Introduction: This research aimed to investigate the effects of using marine probiotics alone and in combination with β-glucan in the diet on digestive enzyme activity, antioxidant defense system activity, and some liver indicators in yellowfin seabream fish.
Materials & Methods: For this study, 450 juvenile fish weighing 13.1±0.49 gr were transferred from Imam Port Aquaculture Station to the college of Veterinary Medicine at Shahid Chamran University in Ahvaz. 4 treatments were considered: Lactobacillus plantarum (108 CFU/g), β-glucan (1%), probiotic+prebiotic, and control. Parameters of the antioxidant defense system such as activity of digestive enzymes including alkaline phosphatase (ALP), trypsin, chymotrypsin, lipase, and α-amylase, were examined. The total bacterial flora and the number of lactic acid bacteria in the gastrointestinal tract of yellowfin seabream (Acanthopagrus arabicus) were also investigated during the 60-day rearing period.
Results: The treatment with Lactobacillus plantarum showed significantly higher levels of some digestive enzyme activities (trypsin and α-amylase) compared to other treatments (p<0.05). The synbiotic treatment had lower values than the probiotic treatment in the experimental parameters. The control group showed higher performance than the prebiotic and synbiotic treatments in certain parameters. It appears that the indigenous bacteria Lactobacillus plantarum alone was able to improve the activity of some digestive enzymes, and lactic acid bacteria flora in yellowfin seabream fish.
Conclusion: The combination of synbiotic Lactobacillus plantarum with β-glucan is not a suitable combination for enhancing the health of yellowfin seabream fish, but due to the ecological and physiological characteristics of this fish species, which have been less studied so far, and also the lack of synergy between β-glucan and Lactobacillus plantarum in the tested fish, further experiments using different prebiotics are needed.