Genetic Comparison of two Spirlin Population Alburnoides eichwaldii (Bloch, 1782) in Tilabad and Shirabad streams (Golestan, Iran) using Microsatellite Markers

Abstract

Spirlin (Alburnoides eichwaldii) is a stream species that has a good abundance in southern basin of Caspian sea, but it is supposed to be extincted in many inland waters of Europe. There hasn’t been any study about genetic diversity of this species yet. In this study, to investigate of Spirlin population structure in Tilabad and Shirabad streams in Golestan province 56 samples (28 samples from each stream) were collected. DNA was extracted by phenol-chloroform method and 6 microsatellite loci were used. Results of this study showed that observed heterozygosity and expected heterozygosity were 0.833 and 0.899, respectively and the Fst value was 0.016 which indicates a low genetic differentiation between the Tilabad, Shirabad and Kaboodwal populations. In examining deviations from Hardy-Weinberg equilibrium, two loci in Tilabad population were balanced and other loci had a significant diviation from Hardy-Weinberg equilibrium (P≤ 0.05). According to the results, the Fst values were 0.020. Most of the loci showed deviation from Hardy-Weinberg equilibrium. Analysis of molecular variance showed there is low genetic variation among populations (1%) and most of the observed variation is within the populations (99%).