Effect of Dietary Encapsulated Lavender Essential Oil on Lymphoied Organ, Liver Enzyme and Antioxidant Property, Intestinal Microbial Population and MUC2 Gene Expression in Broiler Japanese Quails

Abstract

Introduction: Lavender essential oil has antioxidant, antimicrobial, immune system enhancing and digestion and absorption propertie; hence the aim of this study was to determine effect of dietary encapsulated lavender essential oil on lymphoied organ, liver enzyme and antioxidant property, intestinal microbial population and MUC2 gene expression in broiler Japanese quails.
Materials & Methods: The experiment was done with five hundred one days old quail with 5 treatments including the control group and others those consuming encapsulated lavender essential oil levels of 100 200,300 and 400 mg per kg of standard diet with 5 repetitions and 20 quail each. At the end of the study period, the thymus, spleen, and bursa of fabricius relative weight of of two birds from each repetition was examined. Alkaline phosphatase, alanine transaminase and aspartate transaminase and such as glutathione peroxidase, catalase, superoxide dismutase and total antioxidant capacity were examined. Responses to the dinitrochlorobenzene sensitization challenge and mitogen injection of phytohemagglutinin into the left-wing web test were investigated. To investigate the expression of the MUC2 gene, samples from jejunum of two quails were collected. MUC2 gene expression analysis was done using Fermentase company's RNA extraction kit and after RNA extraction and removal of genomic DNA residues and cDNA synthesis for RTPCR reactions, values related to threshold cycle and CTΔ for technical repetitions of MUC2
gene and β-actin It was calculated.
Results: The results of this study showed that encapsulated lavender essential oil supplementation increased thymus, spleen, and bursa of fabricius relative weight compared to the control (P≤0.05). Liver enzyme activities of ALT, AST and ALP decrease and there
were GPX, CAT, SOD and TAC significantly increased instead (P≤0.05). Data showed significant increase in the Lactobacillus colones in contrast to E. coli and Salmonella colonies (P≤0.05). Villi depth and surface, crypth deptht and the number of goblet cells indicate that the beneficial effects of the lavender essential oil on improvment the health status of experimental quails (P≤0.05). MUC2 gene expression increased under influnce of dietary lavender essential oil supplemented diets (P≤0.05).
Conclusion: In conclusion the results of current study showed the beneficial effect of dietary encapsulated lavender essential oil on lymphoied organ relative weights, liver enzyme and antioxidant property, intestinal microbial population, intestinal morphology and
MUC2 gene expression in experimental broiler quails.