شناسایی و جداسازی باکتری های تثبیت کننده ازت در استخرهای پرورش ماهی قزل آلای رنگین کمان شهرستان فیروزکوه

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه زیست شناسی و علوم پایه، واحد رودهن، دانشگاه آزاد اسلامی، رودهن، ایران

10.22034/aej.2021.290971.2565

چکیده

در این مطالعه به منظور شناسایی و جداسازی باکتری های تثبیت کننده ازت در استخرهای پرورش ماهی قزل آلای رنگین کمان ، شصت استخر تکثیر و پرورش ماهی و چهار ایـستگاه نمونـه بـرداری در شهرستان فیروزکوه انتخـاب شـدند. پس از برداشت حجم معینی از آب استخر در محل بیوفیلترها کشت آن ها در محیط کشت نوترینت آگار و LB – Broth بهینه شده و در دمای 25 تا 30 درجه به مدت 5 تا 6 روز انکوبه می گردند و کشت نهایی در محیط کشت اختصاصی نیتروزوموناس و نیتروباکتر انجام شده و کلنی های رشد یافته مشخص شدند. نتایج نشان دادند که بیش ترین تعداد کلنی باکتری جداشده (بیش از 300 تعداد کلنی) در ورودی بیوفیلتر و کم ترین میزان کلنی باکتریایی نیتریفیکاسیون در محیط کشت مربوط به کشت ورودی چاه آب (تعداد 3 کلنی) بود. نتایج این مطالعه روشن ساخت که فعالیت ناشی از پرورش ماهی می‌تواند مقادیر پارامترهای آب مورد استفاده را در طول زمان پرورش ماهی قزل آلا تغییر دهد و باعث نامطلوب شدن برخی از فاکتورهای فیزیکوشیمیایی آب مورد استفاده شود. میزان این تأثیر گذاری تا حد زیادی با توجه به شرایط محیطی به روند پرورش ماهی و شدت فعالیت باکتری‌های نیتروفیکاسیون موجود در آب استخرها دارد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Identification and isolation of nitrogen-fixing bacteria in rainbow trout farms in Firoozkooh city

نویسندگان [English]

  • Leyla Estiri
  • Reza Kazempoor
Department of Biology and Basic Science, Roudehen Branch, Islamic Azad University, Roudehen, Iran
چکیده [English]

In this study, in order to identification and isolation of nitrogen-fixing bacteria in rainbow trout breeding ponds, sixty fish breeding ponds and four sampling stations in Firoozkooh city were selected. After taking a certain volume of pool water in the place of biofilters, their culture is optimized in Nutrition agar and LB-Broth medium and incubated at 25 to 30 degrees for 5 to 6 days. The final culture in Nitrosamines and culture medium Nitrobacteria was performed and the grown colonies were identified. The results showed that the highest number of isolated bacterial colonies (more than 300 colonies) were in the biofilter input and the lowest amount of nitrification bacterial colonies in the culture medium was related to the water well inlet culture (3 colonies).
The results of this study showed that the activity of fish farming can change the values of water parameters used during salmon farming and cause some undesirable physicochemical factors of the water used. The extent of this effect is largely due to environmental conditions to the process of fish farming and the intensity of nitrification bacteria in the pool water.

کلیدواژه‌ها [English]

  • Rainbow trout
  • Water quality parameters
  • Nitrite
  • Microbial contamination
  • Biofilters
  • Nitrate

مراجع

  1. Ghiasi, M., Zorieh Zahra, S.J., Bahonar, A.R., Pourgholam, R., Farabi, S.M.V., Binaii, M. and Saeedi, A.A., 2013. Evaluation of health management of breeding and rearing rainbow trout (Oncorhynchus mykiss) farms in Mazandaran province. Journal of new technologies in aquaculture development. 7(3): 94-100. (In Persian)
  2. Afshari Azad, M.R. and Pouraki, , 2013. Classification and analysis of geomorphic phenomena in the west coast of the Caspian with approach to water level fluctuations. Journal of Geography and Regional Development19(3-4): 177-194. (In Persian)
  3. Food and Agriculture Organization of the United Nations (FAO). 2016. The State of World Fisheries and Aquaculture 2016. Contributing to food security and nutrition for all. Rome. 200 p.
  4. Zimmer, A.M., Nawata, C.M. and Wood, C.M., 2010. Physiological and molecular analysis of the interactive effects of feeding and high environmental ammonia on branchial ammonia excretion and Na+ uptake in freshwater rainbow trout. Journal of Comparative Physiology. 180: 1191-1204.
  5. Kocour Kroupová, H., Valentová, O., Svobodová, Z., Šauer, P. and Máchová, J., 2018. Toxic effects of nitrite on freshwater organisms: a review. Reviews in Aquaculture. 10: 525-542.
  6. Purwono, A.R., Hibbaan, M. and Budihardjo, M.A., 2017. Ammonia-nitrogen (NH3-N) and ammonium-nitrogen (NH4+-N) equilibrium on the process of removing nitrogen by using tubular plastic media. J Mater Environ Sci. 8: 4915-4922.
  7. Wang, Y.B., Li, J.R. and Lin, J., 2008. Probiotics in aquaculture: challenges and outlook. Aquaculture. 281: 1-4.
  8. Almasi Bardmili, B., Alishahi, M. and Javadzadeh, N., 2019. Effect of different levels of Lactobacillus casei as probiotic on survival and growth performance of rainbow trout (Oncorhynchus mykiss) fingerlings. Journal of Animal Environment. 11(2): 259-266. (In Persian)
  9. Rovnyak, G., Andersen, N., Gougoutas, J., Hedberg, A., Kimball, S.D., Malley, M. and Pudzianowski, A., 1991. Active conformation of 1, 4-dihydropyridine calcium entry blockers. Effect of size of 2-aryl substituent on rotameric equilibria and receptor binding. Journal of medicinal chemistry. 34: 2521-2524.
  10. Mahmoudi Khoshdarehgi, , Haji Moradloo, A. and Dastar, B.,  2018. Investigating water quality and growth of Cyprinus carpio fingerlings fed with different protein levels of diet in biofloc system. Journal of Animal Environment. 10(3): 191-198. (In Persian)
  11. Nasrazadani, A., Tahmourespour, A. and Hoodaji, M., 2011. Determination of Bacteria Resistance Threshold to Lead, Zinc and Cadmium in three Industrial Wastewater Samples. Environmental Studies. 36: 56.
  12. Naderi, J.M., Esmaeili, S.A., AhmadI, M.R., Seyfabadi, S.J. and Abdoli, A., 2007. The effects of Trout farm effluents on the water quality parameters of Haraz river. Environmental Sciences. 4 (2):21-36. (In Persian)
  13. Grunditz, C. and Dalhammar, G., 2001. Development of nitrification inhibition assays using pure cultures of Nitrosomonas and Nitrobacter. Water research. 35: 433-440.
  14. McDaniel, N.K., Sugiura, S.H., Kehler, T., Fletcher, J.W., Coloso, R.M., Weis, P. and Ferraris, R.P., 2005. Dissolved oxygen and dietary phosphorus modulate utilization and effluent partitioning of phosphorus in rainbow trout (Oncorhynchus mykiss) aquaculture. Environmental Pollution. 138: 350-357.
  15. Luo, L., Xu, Q., Zhao, Z., Zu, X., Yan, Y. and Jiao, J., 2013. Effect of the adding of carbon resource on water quality of pond aquaculture in bio-flocs technology. Fishery Modernization. 8(10): 512.
  16. Lu, L., Luo, G.Z., Tan, H.X. and Sun, D.C.J.F.M., 2010. Bio-flocs technology application in aquacultural water treatment. 37: 6-10.
  17. Szatkowska, B., 2007. Performance and control of biofilm systems with partial nitritation and anammox for supernatant treatment (Doctoral dissertation, KTH).
  18. Selong, J.H. and Helfrich, L.A., 1998. Impacts of trout culture effluent on water quality and biotic communities in Virginia headwater streams. The Progressive Fish-Culturist. 60: 247-262.
  19. Loch, D.D., West, J.L. and Perlmutter, D.G., 1996. The effect of trout farm effluent on the taxa richness of benthic macroinvertebrates. Aquaculture. 147: 37-55.
  20. Hafezieh, M., Sharifian, M. and Hosseinpour, H., 2015. Biometric comparison of cyst and nauplius of Iranian Artemias. Journal of Aquaculture Development. 9(1): 11-24. (In Persian)
  21. Dehnoei, A., Ganjidoust, H., Ayati, B. and Emtiazi, G., 2012. Comparison of Partial and Complete Nitrification in SBR System for Use in Side Stream Processes. Journal of Water and Wastewater. 23(2): 9-21. (In Persian)
  22. Safari, R., Reyhani Poul, S., Bankehsaz, Zahra, M., Taghavi, J. and Safari, E., 2021. Production of biological silage from wastes of rainbow trout (Oncorhynchus mykiss) using autogenous bacteria and quantitative and qualitative measurement of amino acid and fatty acid profiles of the product. Journal of Aquaculture Sciences. 9(16): 203-212. (In Persian)
  23. Ramin, M., Doustdar, M., Nasrolahzadeh, H. and Vahedi, F., 2014. Physical and chemical factors determination of Mohammadiye channel water in order to aquaculture purposes. Iranian Scientific Fisheries Journal. 22(4): 21-33. (In Persian)
فصلنامه محیط زیست جانوری
دوره 14، شماره 2
تیر 1401
صفحه 347-354

فایل ها

هم رسانی

ارجاع به این مقاله

آمار